Different hyaluronic acid morphology modulates primary articular chondrocyte behavior in hyaluronic acid-coated polycaprolactone scaffolds.

@article{Lebourg2013DifferentHA,
  title={Different hyaluronic acid morphology modulates primary articular chondrocyte behavior in hyaluronic acid-coated polycaprolactone scaffolds.},
  author={Myriam Lebourg and Joaquin Rodenas Rochina and Tiago Sousa and Jo{\~a}o F Mano and J. L. G{\'o}mez Ribelles},
  journal={Journal of biomedical materials research. Part A},
  year={2013},
  volume={101 2},
  pages={518-27}
}
Scaffolds for cartilage tissue engineering should promote both adequate biomechanical environment and chondrogenic stimulation. Hyaluronic acid (HA) has been used in cartilage engineering for its chondrogenic and chondroprotective properties, nevertheless its mechanical properties are limited. Influence of HA microstructure in chondrocyte response has not been addressed yet. In this work, polycaprolactone (PCL) scaffolds were modified using HA following two coating strategies: coating in one… CONTINUE READING

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Chondrocytes were seeded in the scaffolds and cultured in dedifferentiating conditions up to 3 weeks and analyzed using a total DNA assay and sulfated glycosaminoglycan ( sGAG ) determination assay ; cell morphology and extracellular matrix secretion were assessed by electron microscopy as well as immunofluorescent imaging ( collagen I , collagen II , aggrecan , CD44 ) .
Collagen type II seems more prominent in PCL - HA2s ; although PCL - HA2s shows markers for COL II , aggrecan and CD44 , quantitative ECM production is not improved with respect to PCL .
Presence of hyaluronic acid promotes qualitative expression of CD44 surface markers and aggrecan ( more visible in PCL - HA1s than PCL - HA2s ) , whereas in dedifferentiating conditions , expression of CD44 and aggrecan can hardly be detected in pure PCL scaffolds .
Presence of hyaluronic acid promotes qualitative expression of CD44 surface markers and aggrecan ( more visible in PCL - HA1s than PCL - HA2s ) , whereas in dedifferentiating conditions , expression of CD44 and aggrecan can hardly be detected in pure PCL scaffolds .
Collagen type II seems more prominent in PCL - HA2s ; although PCL - HA2s shows markers for COL II , aggrecan and CD44 , quantitative ECM production is not improved with respect to PCL .
Presence of hyaluronic acid promotes qualitative expression of CD44 surface markers and aggrecan ( more visible in PCL - HA1s than PCL - HA2s ) , whereas in dedifferentiating conditions , expression of CD44 and aggrecan can hardly be detected in pure PCL scaffolds .
Presence of hyaluronic acid promotes qualitative expression of CD44 surface markers and aggrecan ( more visible in PCL - HA1s than PCL - HA2s ) , whereas in dedifferentiating conditions , expression of CD44 and aggrecan can hardly be detected in pure PCL scaffolds .
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