Different cation requirements for aggregation of BHK cells and their transformed derivatives.


BHK21 cells singly dissociated by trypsin aggregate in medium with calcium ions, but not in medium with magnesium ions. Their malignant derivatives (pyBHK) induced by polyoma virus, after dissociation with trypsin, aggregate equally well in medium with either calcium or magnesium ions. When EDTA is used for dissociation of cells from culture on a substrate… (More)


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