Different Wnt-5A gene expressions in the renal cell carcinoma GRC-1 cell line during the cell cycle.

Abstract

OBJECTIVE To investigate the gene expression at transcription level of growth factor Wnt-5A in different phase during the cell cycle. METHODS We synchronized the renal cell carcinoma GRC-1 cell line by double thymidine blocks and high-pressure N2O gae methods and amplified Wnt-5A cDNAs from different phase using Semi-quantitative RT-PCR (reverse… (More)

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