Differences in the glycosylation profile of a monoclonal antibody produced by hybridomas cultured in serum-supplemented, serum-free or chemically defined media.

@article{Serrato2007DifferencesIT,
  title={Differences in the glycosylation profile of a monoclonal antibody produced by hybridomas cultured in serum-supplemented, serum-free or chemically defined media.},
  author={J Antonio Serrato and Vanessa Cristina Miguel Hern{\'a}ndez and Sandino Estrada-Mondaca and Laura A. Palomares and Octavio T. Ram{\'i}rez},
  journal={Biotechnology and applied biochemistry},
  year={2007},
  volume={47 Pt 2},
  pages={
          113-24
        }
}
SFM (serum-free medium) is preferred to media containing animal-derived components when culturing mammalian cells for the production of therapeutic recombinant proteins and mAbs (monoclonal antibodies). Nonetheless, eliminating animal-derived components from media can strongly modify culture performance and alter protein glycosylation. In the present study, mAb glycosylation profiles, extracellular exoglycosidase activities, hybridoma growth and mAb production in traditional medium containing… CONTINUE READING
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