Differences in salmon GnRH and chicken GnRH-II contents in discrete brain areas of male and female rainbow trout according to age and stage of maturity.

@article{Okuzawa1990DifferencesIS,
  title={Differences in salmon GnRH and chicken GnRH-II contents in discrete brain areas of male and female rainbow trout according to age and stage of maturity.},
  author={Koichi Okuzawa and Masafumi Amano and Makito Kobayashi and Katsumi Aida and Isao Hanyu and Yoshihisa Hasegawa and Kaoru Miyamoto},
  journal={General and comparative endocrinology},
  year={1990},
  volume={80 1},
  pages={116-26}
}
We have developed sensitive and specific radioimmunoassays (RIA) for salmon gonadotropin-releasing hormone (sGnRH) and chicken GnRH-II (cGnRH-II). Synthetic sGnRH and cGnRH-II(2-10) were conjugated to bovine serum albumin and injected into rabbits to raise specific antisera. The antiserum against sGnRH showed cross-reactivities of 1.58 and 0.08% for cGnRH-II and lamprey GnRH, respectively. The antiserum against cGnRH-II showed cross-reactivities of 0.05 and 0.01% for sGnRH and lamprey GnRH… CONTINUE READING