Differences in glycosaminoglycans synthesized by fibroblast-like cells from chick cornea, heart, and skin.

Abstract

Glycosaminoglycan biosynthesis was studied in primary cultures in vitro to determine if fibroblast-like cells from different individual tissues synthesize the same or different patterns of glycosaminoglycans. Corneas, heart ventricles, and back skins from embryonic chicks were dissociated into cell suspensions. Cornea1 fibroblast-like cells were obtained by simple dissection, whereas heart and skin fibroblast-like cells were separated from nonfibroblastic cell types by their rapid rate of adhesion to plastic substrata. These populations were grown in vitro for 8 days and then were incubated in the continuous presence of o-[6-“HI-glucosamine and H,“%O, for another 2 weeks. All radioactive nutrient medium was collected, as was each cell layer at the end of the experiments. The amount of radioactivity incorporated into each glycosaminoglycan was determined. The patterns of glycosaminoglycans synthesized by the three different fibroblast-like cell populations are clearly distinguishable. Cornea1 populations incorporate twice as high a percentage of radioactivity in heparan sulfate of cell layers as in heparan sulfate of media. Heart populations, on the other hand, incorporate about the same proportion of radioactivity in cell layer heparan sulfate as in that from the media, whereas skin populations show a slightly higher percentage of radioactivity incorporated in cell layer heparan sulfate than in that from media. The proportions of radioactivity incorporated in hyaluronic acid are highest in cornea1 samples, next highest in skin samples, and lowest in heart samples. All three populations release a Zto 3-fold higher proportion of radioactive hyaluronic acid into the media than they retain in their cell layers. Both heart and skin cultures incorporate almost twice as high a percentage of radioactivity in chondroitin 6-sulfate as do cornea1 cultures, whereas the percentage of radioactivity incorporated in chondroitin 4-sulfate is approximately the same in all three populations. Skin cultures incorporate a 3-fold higher percentage of radioactivity in hyaluronic acid released into the media as do heart cultures, yet these skin populations incorporate only half as much radioactivity in cell layer dermatan sulfate as do heart cultures. Analysis of replicate

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@article{Conrad1977DifferencesIG, title={Differences in glycosaminoglycans synthesized by fibroblast-like cells from chick cornea, heart, and skin.}, author={Gary Conrad and Charlotte Hamilton and Ellen E Haynes}, journal={The Journal of biological chemistry}, year={1977}, volume={252 19}, pages={6861-70} }