Differences between standard and high‐sensitivity immunohistology in tissue sections — Comparison of immunoperoxidase staining methods using computerized video image analysis techniques

  title={Differences between standard and high‐sensitivity immunohistology in tissue sections — Comparison of immunoperoxidase staining methods using computerized video image analysis techniques},
  author={Brendon J. Coventry and John Bradley and John M. Skinner},
&NA; High‐sensitivity immunoperoxidase labelling can be achieved using heavy metal enhancement of the di‐amino (DAB) reaction product.1,2 For example nickel chloride combined with DAB improves the sensitivity of the method approximately 7–10 fold. This allows detection of approximately 100–200 molecules on cell surfaces. This has an obvious advantage over standard non‐enhanced DAB methods which detect 1000–2000 molecules under similar conditions.3 This study compares standard and nickel… 

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