Dietary fructo-oligosaccharides and inulin decrease resistance of rats to salmonella: protective role of calcium

  title={Dietary fructo-oligosaccharides and inulin decrease resistance of rats to salmonella: protective role of calcium},
  author={Sandra J M ten Bruggencate and Ingeborg M. J. Bovee-Oudenhoven and Mischa L G Lettink-Wissink and Martijn B. Katan and Roelof van der Meer},
  pages={530 - 535}
Background: We have shown recently that rapid fermentable fructo-oligosaccharides (FOS) decreased resistance of rats towards salmonella. It is not known whether inulin (which is fermented more gradually) has similar effects or whether buffering nutrients can counteract the adverse effects of rapid fermentation. Aims: To compare the effects of dietary inulin and FOS on resistance of rats to Salmonella enterica serovar Enteritidis and to determine whether calcium phosphate counteracts the effects… 

Efficacy of various dietary calcium salts to improve intestinal resistance to Salmonella infection in rats

Calcium phosphate, milk Ca, calcium carbonate and calcium chloride are able to enhance the intestinal resistance to Salmonella in rats and all Ca salts increased resistance towardsSalmonella.

Dietary calcium phosphate strongly impacts gut microbiome changes elicited by inulin and galacto-oligosaccharides consumption

Despite the prebiotic’s substantial difference in chemical structure, sugar composition, oligomer size, and the microbial degradation pathway involved in their utilization, inulin and GOS modulated the gut microbiota very similarly, in a manner that strongly depended on the dietary calcium phosphate level.

Impaired barrier function by dietary fructo-oligosaccharides (FOS) in rats is accompanied by increased colonic mitochondrial gene expression

Altered energy metabolism may underly colonic barrier function disruption due to FOS feeding in rats, indicating that dietary FOS influences intestinal mucosal energy metabolism.

Some putative prebiotics increase the severity of Salmonella enterica serovar Typhimurium infection in mice

In vitro fermentation in monocultures revealed that S. Typhimurium SL1344 is capable of fermenting FOS, beta-glucan and GOS with a corresponding decline in pH, which is in accordance with the increased counts of Salmonella in the organs.

Dietary non-digestible carbohydrates and the resistance to intestinal infections

Stimulating the endogenous microflora by intestinal NDC fermentation is often assumed to be beneficial for intestinal health and resistance to infections, but the work presented in this thesis does not support this concept.

Effects of selected non-digestible dietary carbohydrates on the composition of the large intestinal microbiota and susceptibility to salmonella infections

D diets supplemented with FOS or XOS induced a number of microbial changes in the faecal microbiota of mice, including a significant increase in the Bacteroidetes phylum, the Bacteroides fragilis group and in Bifidobacterium spp.

Certain dietary carbohydrates promote Listeria infection in a guinea pig model, while others prevent it.

Significance of Inulin Fructans in the Human Diet.

It is argued that promising avenues for research are particularly in the areas of energy homeostasis and systemic low-grade inflammation in relation to changes in the composition of the intestinal microbiota.

Dietary calcium decreases but short-chain fructo-oligosaccharides increase colonic permeability in rats

Diet did not influence small-intestinal permeability in rats, likely reflecting relatively slow gut microbiota adaptations, and modulation of mucins and/or microbiota is important for the in vivo effects of dietary Ca and scFOS.

Analysis of the intestinal microbiota of oligosaccharide fed mice exhibiting reduced resistance to Salmonella infection.

D diets supplemented with FOS or XOS induced a number of microbial changes in the faecal microbiota of mice, including a significant increase in the Bacteroidetes phylum, the Bacteroides fragilis group and in Bifidobacterium spp.



Dietary fructo-oligosaccharides dose-dependently increase translocation of salmonella in rats.

Remarkably, FOS dose-dependently increased salmonella numbers in cecal contents and mucosa and caused a major increase in infection-induced diarrhea in rats, and FOS enhanced translocation of Salmonella.

Dietary fructo-oligosaccharides and lactulose inhibit intestinal colonisation but stimulate translocation of salmonella in rats

Despite stimulation of intestinal lactobacilli and bifidobacteria and inhibition of salmonella colonisation, FOS and lactulose significantly enhanced translocation of this pathogen.

Increasing the intestinal resistance of rats to the invasive pathogen Salmonella enteritidis: additive effects of dietary lactulose and calcium.

Extra calcium phosphate added to a lactulose diet improves the resistance to colonisation and translocation of S enteritidis, probably mediated by a calcium induced stimulation of lactULose fermentation by the intestinal microflora and reversion of the lactulOSE mediated increased luminal cytotoxicity, which reduces damage inflicted on the intestinal mucosa.

Effects of two fermentable carbohydrates (inulin and resistant starch) and their combination on calcium and magnesium balance in rats

A combination of different carbohydrates showed synergistic effects on intestinal Ca absorption and balance in rats and confirmed that inulin and resistant starch ingestion led to considerable caecal fermentation in the three experimental groups compared with the control group diet.

Cecal fermentations in rats fed oligosaccharides (inulin) are modulated by dietary calcium level.

There is in the large intestine a system of control of luminal pH, which involves the presence of insoluble Ca and Pi, and in vitro it appears that CaPi is effective in decreasing the solubility of bile salts, chiefly in acidic conditions.

Effect of Dietary Short-Chain Fructooligosaccharides on the Cecal Microflora in Gastrectomized Rats

Dietary Sc-FOS in the gastrectomized rats increased the proportions of Lactobacillus relative to other types of bacteria to levels similar to those seen in healthy normal rats, and decreased the proportion of Bacteroidaceae.

High propionic acid fermentations and mineral accumulation in the cecum of rats adapted to different levels of inulin.

In conclusion, inulin seems very effective in promoting propionic fermentation and in enhancing the calcium content of the large intestine, however, high levels of inulin (greater than 10%) may affect growth in rats and lead to acidic cecal fermentation.

Dietary calcium inhibits the intestinal colonization and translocation of Salmonella in rats.

Dietary calcium improves the colonization resistance and reduces the severity of gut-derived systemic infections, which is probably attributable to its luminal cytoprotective effects.

Calcium in milk and fermentation by yoghurt bacteria increase the resistance of rats to Salmonella infection.

In conclusion, in addition to fermentation by yoghurt bacteria, calcium in milk products strongly enhanced the resistance to salmonella infection by lowering luminal cytolytic activity or diminishing the availability of iron for pathogen growth, or both.

The influence of complex carbohydrates on Salmonella typhimurium colonization, pH, and density of broiler ceca.

The decline of Salmonella infection of broilers fed either refined FOS or LD ceased after dietary additives were discontinued at 5 wk of age, and no consistent effect of cecal pH and density on Salmoneella infection was observed.