Diels-Alder ligation and surface immobilization of proteins.

@article{deAraujo2005DielsAlderLA,
  title={Diels-Alder ligation and surface immobilization of proteins.},
  author={Aline Dantas de Araujo and Jose M. Palomo and Janina Cramer and Maja K{\"o}hn and Hendrik V Schr{\"o}der and Ron Wacker and Christof M. Niemeyer and Kirill Alexandrov and Herbert Waldmann},
  journal={Angewandte Chemie},
  year={2005},
  volume={45 2},
  pages={
          296-301
        }
}
Synthesis of Peptide‐Oligonucleotide Conjugates by Diels‐Alder Cycloaddition in Water
Peptide‐oligonucleotide conjugates incorporating all the nucleobases and trifunctional amino acids are obtained by Diels‐Alder reaction between diene‐modified oligonucleotides (2′‐deoxyribo‐ or
"CHicable" and "Clickable" Copolymers for Network Formation and Surface Modification.
TLDR
Biofunctionalization of the polymer network with alkynylated streptavidin, followed by application of biotin-conjugated antibody and a model analyte, highlights the potential of these surface architectures as a toolbox which can be adapted for diverse bioanalytical applications.
Click Chemistry with Cyclopentadiene.
TLDR
This review discusses the history of the cyclopentadiene cycloaddition as well as applications of cyclopENTadiene click reactions, and focuses on experimental and theoretical studies on the reactivity and stability of cyclanine and cyclopentinadiene derivatives.
Biocompatible conjugation for biodegradable hydrogels as drug and cell scaffolds
TLDR
Recent advances in the field of biodegradable hydrogels crosslinked via biocompatible conjugation are discussed, including both physical and covalent crosslinking, which can be potentially used as drug and cell scaffolds in regenerative medicine applications.
Chemical Synthesis of Native S‐Palmitoylated Membrane Proteins through Removable‐Backbone‐Modification‐Assisted Ser/Thr Ligation
TLDR
The first chemical synthesis of S-palm membrane proteins by reversible backbone modification assisted Ser/Thr Ligation (RBM GABA -assisted STL) is reported, highlighting the importance of developing non-NCL methods for chemical protein synthesis.
Fast surface immobilization of native proteins through catalyst-free amino-yne click bioconjugation†
TLDR
A spontaneous and catalyst-free amino-yne click bioconjugation is used to generate activated ethynyl group functionalized surfaces for fast immobilization of native proteins and cells for highly efficient surface biofunctionalization.
Élucidation des processus de lignification par la stratégie du rapporteur chimique alliée à la microscopie confocale en fluorescence et à la résonance paramagnétique électronique
La lignine est un polymere polyphenolique present au niveau de la paroi vegetale, qui forme avec la cellulose la biomasse lignocellulosique. Cette derniere est impliquee dans de nombreux processus
Chemical Synthesis of Native S-palmitoylated Membrane Proteins through Reversible Backbone Modification Assisted Ser/Thr Ligation.
TLDR
The first chemical synthesis of S-palm membrane proteins by reversible backbone modification assisted Ser/Thr Ligation (RBM GABA -assisted STL) is reported, highlighting the importance of developing non-NCL methods for chemical protein synthesis.
Versatile peptide macrocyclization with Diels-Alder cycloadditions.
TLDR
It is demonstrated that Diels-Alder [4+2] cy-cloadditions can be harnessed for peptide macrocyclization and stabilization within a range of peptide scaffolds and chemical environ-ments.
...
...

References

SHOWING 1-10 OF 31 REFERENCES
Novel Method for the Covalent Immobilization of Oligonucleotides via Diels-Alder Bioconjugation
TLDR
A method for the covalent attachment of diene or maleimide modified oligonucleotides to the coated glass surfaces via aqueous Diels-Alder reactions is presented.
Tissue sulfhydryl groups.
  • G. Ellman
  • Chemistry
    Archives of biochemistry and biophysics
  • 1959
Chemical biology of protein lipidation: semi-synthesis and structure elucidation of prenylated RabGTPases.
TLDR
The structure of the complex provides a structural basis for the ability of RabG DI to inhibit the release of nucleotide by Rab proteins and a molecular basis for understanding a RabGDI mutant that causes mental retardation in humans.
Structure of Rab GDP-Dissociation Inhibitor in Complex with Prenylated YPT1 GTPase
TLDR
The structure of the monoprenylated Ypt1:RabGDI complex provides a structural basis for the ability of RabGDI to inhibit the release of nucleotide by Rab proteins and provides a molecular basis for understanding a RabG DI mutant that causes mental retardation in humans.
Binding of biotin to streptavidin stabilizes intersubunit salt bridges between Asp61 and His87 at low pH.
  • B. Katz
  • Chemistry
    Journal of molecular biology
  • 1997
TLDR
In addition to the established role of biotin in tetramer stabilization by direct mediation of intersubunit interactions at the weak interface through contact with Trp120, biotin may enhance tetramer stability at the strong interface more indirectly by ordering loop residues.
Methods and strategies of peptide ligation.
TLDR
This review focuses on the concept, methods, and strategies of orthogonal peptide ligation, an amino terminal specific method to couple chemically unprotected peptides or proteins derived from synthetic or biosynthetic sources.
...
...