Dichotomous but stringent substrate selection by the dual-function Cdk7 complex revealed by chemical genetics

@article{Larochelle2006DichotomousBS,
  title={Dichotomous but stringent substrate selection by the dual-function Cdk7 complex revealed by chemical genetics},
  author={St{\'e}phane Larochelle and Jasmin Batliner and Matthew J. Gamble and Nora M Barboza and Brian C Kraybill and Justin D. Blethrow and Kevan M. Shokat and Robert P Fisher},
  journal={Nature Structural &Molecular Biology},
  year={2006},
  volume={13},
  pages={55-62}
}
Cdk7 performs two essential but distinct functions as a CDK-activating kinase (CAK) required for cell-cycle progression and as the RNA polymerase II (Pol II) CTD kinase of general transcription factor IIH. To investigate the substrate specificity underlying this dual function, we created an analog-sensitive (AS) Cdk7 able to use bulky ATP derivatives. Cdk7-AS–cyclin H–Mat1 phosphorylates ∼10–15 endogenous polypeptides in nuclear extracts. We identify seven of these as known and previously… CONTINUE READING

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Identification and characterization of a novel cell cycle - regulated internal ribosome entry site

  • S. Cornelis
  • J . Am . Chem . Soc .
  • 2002

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