Dicer is dispensable for asymmetric RISC loading in mammals.

@article{Betancur2012DicerID,
  title={Dicer is dispensable for asymmetric RISC loading in mammals.},
  author={Juan Guillermo Betancur and Yukihide Tomari},
  journal={RNA},
  year={2012},
  volume={18 1},
  pages={24-30}
}
In flies, asymmetric loading of small RNA duplexes into Argonaute2-containing RNA-induced silencing complex (Ago2-RISC) requires Dicer-2/R2D2 heterodimer, which acts as a protein sensor for the thermodynamic stabilities of the ends of small RNA duplexes. However, the mechanism of small RNA asymmetry sensing in mammalian RISC assembly remains obscure. Here, we quantitatively examined RISC assembly and target silencing activity in the presence or absence of Dicer in mammals. Our data show that… CONTINUE READING

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