Dicer-deficient mouse embryonic stem cells are defective in differentiation and centromeric silencing.

@article{Kanellopoulou2005DicerdeficientME,
  title={Dicer-deficient mouse embryonic stem cells are defective in differentiation and centromeric silencing.},
  author={Chryssa Kanellopoulou and Stefan A Muljo and Andrew L Kung and Shridar Ganesan and Ronny Drapkin and Thomas Jenuwein and David Livingston and Klaus Rajewsky},
  journal={Genes & development},
  year={2005},
  volume={19 4},
  pages={
          489-501
        }
}
Dicer is the enzyme that cleaves double-stranded RNA (dsRNA) into 21-25-nt-long species responsible for sequence-specific RNA-induced gene silencing at the transcriptional, post-transcriptional, or translational level. We disrupted the dicer-1 (dcr-1) gene in mouse embryonic stem (ES) cells by conditional gene targeting and generated Dicer-null ES cells. These cells were viable, despite being completely defective in RNA interference (RNAi) and the generation of microRNAs (miRNAs). However, the… CONTINUE READING

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