Dicer-deficient mouse embryonic stem cells are defective in differentiation and centromeric silencing.

@article{Kanellopoulou2005DicerdeficientME,
  title={Dicer-deficient mouse embryonic stem cells are defective in differentiation and centromeric silencing.},
  author={Chryssa Kanellopoulou and Stefan A. Muljo and Andrew L. Kung and Shridar Ganesan and Ronny Drapkin and Thomas Jenuwein and David M Livingston and Klaus Rajewsky},
  journal={Genes \& development},
  year={2005},
  volume={19 4},
  pages={
          489-501
        }
}
Dicer is the enzyme that cleaves double-stranded RNA (dsRNA) into 21-25-nt-long species responsible for sequence-specific RNA-induced gene silencing at the transcriptional, post-transcriptional, or translational level. We disrupted the dicer-1 (dcr-1) gene in mouse embryonic stem (ES) cells by conditional gene targeting and generated Dicer-null ES cells. These cells were viable, despite being completely defective in RNA interference (RNAi) and the generation of microRNAs (miRNAs). However, the… 
Characterization of Dicer-deficient murine embryonic stem cells.
Dicer is an RNase III-family nuclease that initiates RNA interference (RNAi) and related phenomena by generation of the small RNAs that determine the specificity of these gene silencing pathways. We
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The use of genome editing to generate inactivating deletion mutations in all three copies of the dicer (hdcr) gene present in the human cell line 293T demonstrates that the mechanisms underlying the accurate processing of pre-miRNAs are largely, but not entirely, conserved between mammalian and insect cells.
Dicer deletion and short RNA expression analysis in mouse embryonic stem cells
TLDR
A detailed comparison of short RNAs expressed in mouse embryonic stem cells with and without the ribonuclease Dicer strongly suggests that miRNAs are the sole regulatory molecules that function through the RNAi pathway in ES cells, raising the possibility that mammalian mi RNAs may in certain contexts function to silence repeating genomic elements in addition to protein-coding genes.
RNAi-Dependent and Independent Control of LINE1 Accumulation and Mobility in Mouse Embryonic Stem Cells
TLDR
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A Retrotransposon-Driven Dicer Isoform Directs Endogenous Small Interfering RNA Production in Mouse Oocytes
TLDR
The alternative Dicer isoform, whose phylogenetic origin demonstrates evolutionary plasticity of RNA-silencing pathways, is the main determinant of endogenous RNAi activity in the mouse female germline.
Dicer promotes genome stability via the bromodomain transcriptional co-activator Brd4
TLDR
It is demonstrated that deletion of specific residues in Brd4 rescue genome instability defects of Dicer1-/- in both mammalian cells and fission yeast, implicating Dicer in coordinating transcription and replication of satellite repeats.
RNA interference in mammalian DNA methylation.
  • J. Mann, D. Mattiske
  • Biology, Medicine
    Biochemistry and cell biology = Biochimie et biologie cellulaire
  • 2012
TLDR
It is considered it equally likely that the defects in DNA methylation that can be observed in DICER1-deficient embryonic stem cells are the result of nonspecific effects related to RNAi loss aside from reduced DNMT activity.
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