Dibasic amines as competitive ions improve the resolution between polyanionic nucleotides.

  title={Dibasic amines as competitive ions improve the resolution between polyanionic nucleotides.},
  author={L. Callahan and N. Schwartz and K. Ng},
  journal={Analytical biochemistry},
  volume={196 2},
Aliphatic diamines when used as single ion pairing reagents were capable of resolving 3'-,5'- and 2'-,5'- nucleotidyl diphosphates from one another while conventional ion pairing reagents did not separate these positional isomers. The use of 1,2-diamines resulted in the greatest resolution while increasing spacing between the amino groups progressively reduced the resolution while increasing the retention volume. A competitive ion pairing system was also developed using triethylamine as an… Expand
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