Diameter measurement from images of fluorescent cylinders embedded in tissue

  title={Diameter measurement from images of fluorescent cylinders embedded in tissue},
  author={Marijn P. Rolf and Ren{\'e} ter Wee and Ton G. van Leeuwen and Jos A. E. Spaan and Geert J. Streekstra},
  journal={Medical & Biological Engineering & Computing},
Absorption and scattering of light by tissue as well as limitations in the resolution of the optical system influence the appearance of tissue embedded objects in fluorescence images and may reduce the accuracy of measurements from these images. Although the principles of light scattering in tissue and optical resolution are well known, the interplay between the two in fluorescence imaging in an imaging cryomicrotome is not well understood. In this paper we present and investigate an image… CONTINUE READING


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