Diallyl disulfide ameliorates methotrexate-induced nephropathy in rats: Molecular studies and network pharmacology analysis.

  title={Diallyl disulfide ameliorates methotrexate-induced nephropathy in rats: Molecular studies and network pharmacology analysis.},
  author={Emad H. M. Hassanein and Wafaa R. Mohamed and Marwa M. Khalaf and Abdel-Gawad S. Shalkami and Ahmed M. Sayed and Ramadan A. M. Hemeida},
  journal={Journal of food biochemistry},
Methotrexate (MTX) is a promising chemotherapeutic agent. Its medical use is limited by induced nephropathy. Our study was designed to explore the reno-protective effect of diallyl disulfide (DADS), an organosulfur compound of garlic oil, on MTX-induced nephropathy. Adult rats were randomly divided into 4 groups; normal control, DADS (50 mg kg-1  day-1 , p.o.), MTX (20 mg/kg, i.p.) and DADS+MTX. DADS significantly decreased serum creatinine, urea, uric acid, and albumin levels with an… 
5 Citations

Protective Effect of Pycnogenol against Methotrexate-Induced Hepatic, Renal, and Cardiac Toxicity: An In Vivo Study

Analysis of the molecular and microscopic basis of MTX-induced toxicity in different organs and the possible protective effect of pycnogenol, a polyphenolic component extracted from the bark of P. pinaster, reveal that pyCNogenol successfully ameliorated oxidative damage and reduced toxicity, inflammatory response, and histological markers induced by methotrexate treatment.

The Effects of Antioxidant Nutraceuticals on Cellular Sulfur Metabolism and Signaling.

It is proposed that nutraceutical-specific targeting of RSS metabolism will lead to the design and development of even more efficacious antioxidant therapeutic strategies.

Biological Functions of Diallyl Disulfide, a Garlic-Derived Natural Organic Sulfur Compound

This review systematically evaluated the biological functions of DADS and discussed the underlying molecular mechanisms of these functions, hoping that this review provides guidance and insight into the current literature and enables future research and the development of D ADS for intervention and treatment of multiple diseases.

Tris(methylthio)methane produced by Mortierella hyalina affects sulfur homeostasis in Arabidopsis

Microbial volatiles are important factors in symbiotic interactions with plants. Mortierella hyalina is a beneficial root-colonizing fungus with a garlic-like smell, and promotes growth of



Diallyl disulfide attenuates acetaminophen-induced renal injury in rats

The results suggest that DADS prevents AAP-induced acute nephrotoxicity, and that KIM-1 and NGAL may be useful biomarkers for the detection and monitoring of acute kidney injury associated with AAP exposure.

Renoprotective effects of umbelliferone on methotrexate-induced renal injury through regulation of Nrf-2/Keap-1, P38MAPK/NF-κB, and apoptosis signaling pathways.

Rebamipide potentially mitigates methotrexate‐induced nephrotoxicity via inhibition of oxidative stress and inflammation: A molecular and histochemical study

Rebamipide treatment attenuated the reduction in glomerular size, the widening of the capsular spaces, and the tubular cell damage due to MTX administration, indicating the potential of REB as adjuvant therapy to prevent nephrotoxicity in patients receiving MTX treatment.

Neutrophil Infiltration and Oxidative Stress May Play a Critical Role in Methotrexate-Induced Renal Damage

Evidence is provided for the role of neutrophil infiltration and oxidative stress in MTX-induced renal damage and administration of inhibitors of myeloperoxidase or scavenging hypochlorous acid by supplementation with antioxidants as an adjuvant therapy may be promising in alleviating the renal side effect of MTX.

Formononetin Upregulates Nrf2/HO-1 Signaling and Prevents Oxidative Stress, Inflammation, and Kidney Injury in Methotrexate-Induced Rats

FN prevents MTX-induced AKI by activating Nrf2/HO-1 signaling and attenuates oxidative damage and inflammation and may represent an effective adjuvant that can prevent MTX nephrotoxicity, pending further mechanistic studies.

Protective effect of pentoxyfilline in renal toxicity after methotrexate administration.

The increased level of tissue MDA and serum TNF-alpha level together may be suggested that the underlying mechanism is related to direct toxicity of MTX rather than blockage in folate synthesis in kidneys.

Protective effects of diallyl disulfide on carbon tetrachloride‐induced hepatotoxicity through activation of Nrf2

The results indicate that the protective effects of DADS against CCl4‐induced hepatotoxicity possibly involve mechanisms related to its ability to induce antioxidant or detoxifying enzymes by activating Nrf2 and block metabolic activation of CCl 4 by suppressing CYP2E1.

Hepatoprotective effect of rebamipide against methotrexate-induced hepatic intoxication: role of Nrf2/GSK-3β, NF-κβ-p65/JAK1/STAT3, and PUMA/Bax/Bcl-2 signaling pathways

The outcomes of the present study showed REB’s ability to protect from hepatic injury induced by MTX, possibly through its antioxidant, anti-inflammatory, and anti-apoptotic properties.