[Diagnostic value of leukocytic alteration test (in vitro) for drug-induced allergy in a therapist's practice].

Abstract

The laboratory diagnostic process of drug intolerance is a clinical challenge. The allergic leukocytic alteration test (ALAT) was used to detect drug intolerance and to estimate its informative value. Three hundred and eighty-seven patients with various manifestations of drug allergy were examined (1227 studies). The test was carried out in vitro using heparinized blood, calculated with reference with 1 ml per drug and incubated at 37 degrees C for 1.5 hours. Smears were prepared, stained by the Romanovsky-Giemsa method, and examined by light microscopy. A hundred cells (neutrophils) were scanned to evaluate allergic alteration. When the affected neutrophils were more than 10%, the test was considered as positive. The findings suggest that ALAT reveals intolerance of different groups of drugs with a high degree of significance and may be proposed for the diagnosis of drug allergy as it is of great informative value, safe to a patient, and allows a large number of drugs to be tested.

Cite this paper

@article{Khalimova2008DiagnosticVO, title={[Diagnostic value of leukocytic alteration test (in vitro) for drug-induced allergy in a therapist's practice].}, author={Zamira Khalimova and {\'E} M {\'E}sedov and L B Ashurbekova}, journal={Klinicheskaia laboratornaia diagnostika}, year={2008}, volume={12}, pages={23-4, 32-3} }