Diagnostic value of PCR for detection of Borrelia burgdorferi in skin biopsy and urine samples from patients with skin borreliosis.

@article{Brettschneider1998DiagnosticVO,
  title={Diagnostic value of PCR for detection of Borrelia burgdorferi in skin biopsy and urine samples from patients with skin borreliosis.},
  author={Susan Brettschneider and Harald Bruckbauer and Norbert Klugbauer and Hanns Hofmann},
  journal={Journal of clinical microbiology},
  year={1998},
  volume={36 9},
  pages={2658-65}
}
Skin biopsies of 36 patients with erythema migrans and acrodermatitis chronica atrophicans (ACA) before therapy and those of 8 patients after therapy were examined for Borrelia burgdorferi DNA by PCR. Skin biopsies of 27 patients with dermatological diseases other than Lyme borreliosis and those of 10 healthy persons were examined as controls. Two different primer sets targeting 23S rRNA (PCR I) and 66-kDa protein (PCR II) genes were used. PCR was performed with freshly frozen tissue (FFT) and… CONTINUE READING