Diagnostic tool for the identification of MLL rearrangements including unknown partner genes.

@article{Meyer2005DiagnosticTF,
  title={Diagnostic tool for the identification of MLL rearrangements including unknown partner genes.},
  author={Claus Meyer and Bjoern Schneider and Martin Reichel and Sieglinde Angermueller and Sabine Strehl and Susanne Schnittger and Claudia Schoch and M W Jansen and Jacques J M van Dongen and Rob Pieters and Oskar A. Haas and Theodor Dingermann and Thomas Klingebiel and Rolf Marschalek},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2005},
  volume={102 2},
  pages={
          449-54
        }
}
Approximately 50 different chromosomal translocations of the human MLL gene are currently known and associated with high-risk acute leukemia. The large number of different MLL translocation partner genes makes a precise diagnosis a demanding task. After their cytogenetic identification, only the most common MLL translocations are investigated by RT-PCR analyses, whereas infrequent or unknown MLL translocations are excluded from further analyses. Therefore, we aimed at establishing a method that… Expand
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References

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Panhandle PCR: a technical advance to amplify MLL genomic translocation breakpoints
TLDR
Panhandle PCR is a straightforward method that represents a technical advance in MLL genomic breakpoint cloning and offers the advantage of having specificity for the strand of interest at both primer annealing sites without requiring specific primers for the many partner genes of MLL. Expand
Biased distribution of chromosomal breakpoints involving the MLL gene in infants versus children and adults with t(4;11) ALL
TLDR
Derivative chromosomes of 40 patients diagnosed with t(4;11) acute lymphoblastic leukemia were analysed on the genomic DNA level, revealing significant differences in the distribution of chromosomal breakpoints and leading to the definition of two hotspot areas within the MLL breakpoint cluster region. Expand
Molecular cytogenetic analysis of 10;11 rearrangements in acute myeloid leukemia
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A detailed molecular cytogenetic analysis of MLL-MLLT10 positive 10;11 rearrangements in two patients is described, observing an as yet unreported chromosomal mechanism with at least four breakpoints, leading to M LL-M LLT10 gene fusion in a 24-year-old male. Expand
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TLDR
This study shows that panhandle PCR is an effective method for cloning MLL genomic breakpoints in treatment-related leukemias, and analysis of additional pediatric cases will determine whether breakpoint distribution deviates from the predilection for 3' distribution in the bcr that has been found in adult cases. Expand
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TLDR
This case is the first to provide direct cytogenetic evidence for the salient nature of the MLL/SEPTIN6 rearrangement, and suggested that MLL and SEPTin6 reside on their respective chromosome loci in reverse orientation, that is, centromere‐to‐telomere and telomere‐ to‐centromere, respectively. Expand
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TLDR
The results of Southern blotting in conjunction with fluorescence in situ hybridization suggest that the MLL‐CBL fusion was the result of an interstitial deletion. Expand
Panhandle Polymerase Chain Reaction Amplifies MLL Genomic Translocation Breakpoint Involving Unknown Partner Gene
We used a new approach called panhandle polymerase chain reaction (PCR) to clone an MLL genomic translocation breakpoint in a case of acute lymphoblastic leukemia of infancy in which karyotypeExpand
Panhandle and reverse-panhandle PCR enable cloning of der(11) and der(other) genomic breakpoint junctions of MLL translocations and identify complex translocation of MLL, AF-4, and CDK6
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  • Biology, Medicine
  • Proceedings of the National Academy of Sciences of the United States of America
  • 2002
TLDR
This work used panhandle PCR to clone the der(11) genomic breakpoint junction in three leukemias with t(4;11) and devised reverse-panhandle PCR for cloning breakpoint junctions of the other derivative chromosomes, sequences of which are germane to understanding the MLL translocation process. Expand
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