Diagnostic reverse-transcription polymerase chain reaction kit for filoviruses based on the strain collections of all European biosafety level 4 laboratories.

@article{Panning2007DiagnosticRP,
  title={Diagnostic reverse-transcription polymerase chain reaction kit for filoviruses based on the strain collections of all European biosafety level 4 laboratories.},
  author={Marcus Panning and Thomas Laue and Stephan Olschlager and Markus Eickmann and Stephan Becker and Sabine Raith and M C Georges Courbot and M. T. Nilsson and R. T. Venu Gopal and {\AA}ke Lundkvist and Antonino Di Caro and David Brown and Hermann v. Meyer and Graham Lloyd and Beate M Kummerer and Stephan Gunther and Christian Drosten},
  journal={The Journal of infectious diseases},
  year={2007},
  volume={196 Suppl 2},
  pages={S199-204}
}
A network of European biosafety level 4 laboratories has designed the first industry-standard molecular assay for all filoviruses species, based on the strain collections of all participants. It uses 5 optimized L gene primers and 3 probes, as well as an internal control with a separate detection probe. Detection limits (probit analysis, 95% detection chance) were as follows: Zaire ebolavirus, 487 copies/mL of plasma; Sudan ebolavirus Maleo, 586 copies/mL; Sudan ebolavirus Gulu, 1128 copies/mL… CONTINUE READING

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Clinical infectious diseases : an official publication of the Infectious Diseases Society of America • 2006
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