Diagnostic profile on the IFA 40: HEp-20-10 - an immunofluorescence test for reliable antinuclear antibody screening.

@article{Rohwder2015DiagnosticPO,
  title={Diagnostic profile on the IFA 40: HEp-20-10 - an immunofluorescence test for reliable antinuclear antibody screening.},
  author={Edda Rohw{\"a}der and Michael Locke and Johanna Fraune and Kai Fechner},
  journal={Expert review of molecular diagnostics},
  year={2015},
  volume={15 4},
  pages={451-62}
}
Indirect immunofluorescence assay is the recommended gold standard to test for antinuclear antibodies (ANA), which are important biomarkers for systemic rheumatic autoimmune diseases. It is internationally accepted that indirect immunofluorescence assay ANA screening is most sensitive on human epithelial (HEp-2) cells. The cells present a multitude of antigens that display distinguishable localization patterns in interphase and mitotic cells in indirect immunofluorescence analysis. Here, we… CONTINUE READING