Diagnostic multiplex PCR for toxin genotyping of Clostridium perfringens isolates.

  title={Diagnostic multiplex PCR for toxin genotyping of Clostridium perfringens isolates.},
  author={Christoph Georg Baums and Ulrich Schotte and Gunter Amtsberg and Ralph Goethe},
  journal={Veterinary microbiology},
  volume={100 1-2},
In this study we provide a protocol for genotyping Clostridium perfringens with a new multiplex PCR. This PCR enables reliable and specific detection of the toxin genes cpa, cpb, etx, iap, cpe and cpb2 from heat lysed bacterial suspensions. The efficiency of the protocol was demonstrated by typing C. perfringens reference strains and isolates from veterinary bacteriological routine diagnostic specimens. 
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