Diagnostic microRNA markers to screen for sporadic human colon cancer in blood.

@article{Ahmed2012DiagnosticMM,
  title={Diagnostic microRNA markers to screen for sporadic human colon cancer in blood.},
  author={Farid E. Ahmed and Nancy C Amed and Paul Vos and Chris Bonnerup and James Norman Atkins and Michelle Casey and Gerard J Nuovo and Wade Naziri and John E. Wiley and Ron R. Allison},
  journal={Cancer genomics & proteomics},
  year={2012},
  volume={9 4},
  pages={179-92}
}
We carried out this study to present proof-of-principal application, showing that by using a global microarray expression analysis, followed by quantitative stem-loop reverse transcriptase in conjunction with TaqMan® polymerase chain reaction (PCR) analysis of micro(mi)RNA genes, on limited number of plasma and tissue samples obtained from 20 individuals (five healthy, five TNM stage 0-1 colon cancer, five stage 2 and five stage 3), we were able to quantitatively monitor miRNA changes at the… CONTINUE READING
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