Diagnostic evaluation of a real-time RT-PCR assay for routine diagnosis of classical swine fever in wild boar.

@article{Depner2006DiagnosticEO,
  title={Diagnostic evaluation of a real-time RT-PCR assay for routine diagnosis of classical swine fever in wild boar.},
  author={Klaus Robert Depner and Claudia Bunzenthal and B Heun-M{\"u}nch and G{\"u}nter Strebelow and Bernd Hoffmann and Martin Beer},
  journal={Journal of veterinary medicine. B, Infectious diseases and veterinary public health},
  year={2006},
  volume={53 7},
  pages={317-20}
}
The aim of the study was to evaluate a real-time reverse transcription-polymerase chain reaction (RT-PCR) assay for routine diagnosis of classical swine fever (CSF) in wild boar by using 1000 spleen homogenates that were tested previously negative by virus isolation and 26 homogenates from which CSF virus could be isolated. All 26 positive samples in the virus isolation assay also were found to be positive in real-time RT-PCR. Additionally further 10 samples were detected by real-time RT-PCR… CONTINUE READING

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