Diagnostic PCR with Leishmania donovani specificity using sequences from the variable region of kinetoplast minicircle DNA.

@article{Singh1999DiagnosticPW,
  title={Diagnostic PCR with Leishmania donovani specificity using sequences from the variable region of kinetoplast minicircle DNA.},
  author={Neeloo Singh and Martin David Curran and A K Rastogil and Derek Middleton and Shyam Sundar},
  journal={Tropical medicine & international health : TM & IH},
  year={1999},
  volume={4 6},
  pages={448-53}
}
Kala azar or visceral leishmaniasis, a parasitic disease caused by Leishmania donovani, is presently causing an epidemic in the eastern region of India. Diagnosis of kala azar is often complicated. We developed a pair of oligonucleotides suitable as primers from the variable region of a predominant sequence class of minicircles of L. donovani. These primers were used in a nonisotopic polymerase chain reaction and found to be highly specific for the parasites of L. donovani complex. Using these… CONTINUE READING

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