Diagnosis of visceral leishmaniasis: the sensitivities and specificities of traditional methods and a nested PCR assay.

@article{Gatti2004DiagnosisOV,
  title={Diagnosis of visceral leishmaniasis: the sensitivities and specificities of traditional methods and a nested PCR assay.},
  author={Simonetta Gatti and Maria Gramegna and Catherine Klersy and S. Madama and Antonella Bruno and Roberta Maserati and A. M. Bernuzzi and C. Cevini and Massimo Scaglia},
  journal={Annals of tropical medicine and parasitology},
  year={2004},
  volume={98 7},
  pages={
          667-76
        }
}
In the present study, 67 patients suspected to be cases of visceral leishmaniasis (VL) were each checked for leishmanial infection by the microscopical evaluation of various biological specimens, in-vitro culture, serology and an assay based on nested PCR. Most (35) of the subjects were immunocompetent (IC) but 32 were immunodeficient (ID) as the result of HIV infection (18 cases), treatment to prevent transplanted organs being rejected (six) or haematological malignancies (eight). Forty-one… CONTINUE READING

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