Diagnosis of swine trichinosis by enzyme-linked immunosorbent assay (ELISA) using an excretory--secretory antigen.

@article{Gamble1983DiagnosisOS,
  title={Diagnosis of swine trichinosis by enzyme-linked immunosorbent assay (ELISA) using an excretory--secretory antigen.},
  author={Howard R Gamble and Wesley R. Anderson and Charles E. Graham and Kenneth Darwin Murrell},
  journal={Veterinary parasitology},
  year={1983},
  volume={13 4},
  pages={
          349-61
        }
}
An excretory--secretory (ES) antigen was used in a serodiagnostic enzyme-linke immunosorbent assay (ELISA) for swine trichinosis. ELISA procedures included a double-antibody test, using either an anti-swine IgG or a protein A enzyme conjugate, and a triple-antibody test using a pig IgG heavy-chain specific second antibody with a conjugated third antibody. The ES antigen was effective in eliminating all false-positive reactivity in sera from farm-raised hogs. The triple-antibody procedure was… CONTINUE READING
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