Diagnosis of primary liver cancer using lectin affinity chromatography of serum alkaline phosphatase.

@article{Lu1997DiagnosisOP,
  title={Diagnosis of primary liver cancer using lectin affinity chromatography of serum alkaline phosphatase.},
  author={Y Lu and Quan Lu and Hui Li Chen},
  journal={Journal of experimental & clinical cancer research : CR},
  year={1997},
  volume={16 1},
  pages={75-80}
}
Serum alkaline phosphatase (sALP) can be separated into unbound liver type (L-ALP) and bound bone type (B-ALP) by means of WGA affinity chromatography. The L-ALP from the sera of normal adults and various liver diseases was found to show different chromatographic behaviours on DSA affinity column with multiple peaks of ALP activity after the L-ALP was treated with neuraminidase to remove the terminal sialic acids on the sugar chain of L-ALP. The L-ALP from normal sera contained no bound… CONTINUE READING
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