Diagnosis of meningococcal meningitis by broad-range bacterial PCR with cerebrospinal fluid.

@article{Kotilainen1998DiagnosisOM,
  title={Diagnosis of meningococcal meningitis by broad-range bacterial PCR with cerebrospinal fluid.},
  author={Pirkko Kotilainen and Jari Jalava and Olli H. Meurman and O. P. Lehtonen and Eija Rintala and O P Sepp{\"a}l{\"a} and Erkki Eerola and Simo Nikkari},
  journal={Journal of clinical microbiology},
  year={1998},
  volume={36 8},
  pages={2205-9}
}
We used broad-range bacterial PCR combined with DNA sequencing to examine prospectively cerebrospinal fluid (CSF) samples from patients with suspected meningitis. Fifty-six CSF samples from 46 patients were studied during the year 1995. Genes coding for bacterial 16S and/or 23S rRNA genes could be amplified from the CSF samples from five patients with a clinical picture consistent with acute bacterial meningitis. For these patients, the sequenced PCR product shared 98.3 to 100% homology with… CONTINUE READING

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