Diagnosis of human parvovirus infection by dot-blot hybridization using cloned viral DNA.

@article{Anderson1985DiagnosisOH,
  title={Diagnosis of human parvovirus infection by dot-blot hybridization using cloned viral DNA.},
  author={Marie Anderson and Susan E. Jones and Anthony Charles Minson},
  journal={Journal of medical virology},
  year={1985},
  volume={15 2},
  pages={
          163-72
        }
}
The human parvovirus can be detected in serum by the immunological techniques of immune electron microscopy (IEM), counterimmunoelectrophoresis (CIE), and radioimmunoassay (RIA). A portion of the genome of this virus has been cloned in pAT153 and used as a 32P-labelled probe in dot-blot hybridization assays to detect parvovirus DNA in serum specimens. This test proved a highly sensitive means of detecting virus in microlitre volumes of serum, giving positive results for samples containing 0.5… CONTINUE READING

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