Diagnosis of foot-and mouth disease by real time reverse transcription polymerase chain reaction under field conditions in Brazil

@article{Paixo2008DiagnosisOF,
  title={Diagnosis of foot-and mouth disease by real time reverse transcription polymerase chain reaction under field conditions in Brazil},
  author={Tatiane A Paix{\~a}o and Alcina V. Carvalho Neta and Naimes O Paiva and Jorge R Reis and Meirivan S Barbosa and Claudia V Serra and Ren{\'e} Ribeiro Silva and Tammy R. Beckham and Barbara M. Martin and Neville P. Clarke and Leslie Garry Adams and Renato Lima Santos},
  journal={BMC Veterinary Research},
  year={2008},
  volume={4},
  pages={53 - 53}
}
BACKGROUND Foot-and-mouth disease (FMD) is an economically important and highly contagious viral disease that affects cloven-hoofed domestic and wild animals. Virus isolation and enzyme-linked immunosorbent assay (ELISA) are the gold standard tests for diagnosis of FMD. As these methods are time consuming, assays based on viral nucleic acid amplification have been developed. RESULTS A previously described real-time reverse transcriptase polymerase chain reaction (RT-PCR) assay with high… CONTINUE READING