Diagnosis of bubonic plague by PCR in Madagascar under field conditions.

@article{Rahalison2000DiagnosisOB,
  title={Diagnosis of bubonic plague by PCR in Madagascar under field conditions.},
  author={Lila Rahalison and Elie Jeanne Vololonirina and Mahery Ratsitorahina and Suzanne Chanteau},
  journal={Journal of clinical microbiology},
  year={2000},
  volume={38 1},
  pages={260-3}
}
The diagnostic value of a PCR assay that amplifies a 501-bp fragment of the Yersinia pestis caf1 gene has been determined in a reference laboratory with 218 bubo aspirates collected from patients with clinically suspected plague managed in a regional hospital in Madagascar. The culture of Y. pestis and the detection of the F1 antigen (Ag) by enzyme-linked immunosorbent assay (ELISA) were used as reference diagnostic methods. The sensitivity of PCR was 89% (57 of 64) for the Y. pestis-positive… CONTINUE READING

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