Diagnosis of Gaucher's disease in cultured skin fibroblasts and leucocytes

@article{Butterworth1978DiagnosisOG,
  title={Diagnosis of Gaucher's disease in cultured skin fibroblasts and leucocytes},
  author={J. B. Butterworth and D. M. Broadhead},
  journal={Journal of Inherited Metabolic Disease},
  year={1978},
  volume={1},
  pages={111-113}
}
Washing skin fibroblasts or leucocytes in 0.25 mol/l sucrose increases the activity ofβ-glucosidase at acid pH. This effect is primarily due to removal of low levels of sodium chloride, which inhibit acidβ-glucosidase. A secondary factor for skin fibroblasts is the removal of residual phosphate buffer pH 7.3 used to wash the cells following trypsinization. As theβ-glucosidase activity of water-lysed leucocytes is higher at acid pH than that of a saline suspension of leucocytes, the former are… CONTINUE READING