• Corpus ID: 13078074

Diagnosis of Eimeriosis in Cattle by ELISA Using Partially Purified Antigen

@inproceedings{Toaleb2011DiagnosisOE,
  title={Diagnosis of Eimeriosis in Cattle by ELISA Using Partially Purified Antigen},
  author={Nagwa I. Toaleb and Faragalla M. El-Moghazy and Soad E. Hassan},
  year={2011}
}
2 Abstract: Chromatographic analysis of Eimeria sp. crude antigen was done by Sephacryl S-200 column. Four fractions were isolated by this approach. Comparative evaluation of the immunogenic binding activities of isolated fractions by ELISA proved the potency of fraction number one (F1) over the other three ones. Characterization of the isolated fraction (F1) by SDS polyacrylamide gel electrophoresis showed that the fraction consists of 3 bands of 225, 198, 114.5 KDa. Only one band of molecular… 

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