Diagnosis and quantification of Theileria sergenti using TaqMan PCR.

  title={Diagnosis and quantification of Theileria sergenti using TaqMan PCR.},
  author={Wooseog Jeong and Chang Hee Kweon and Seung Won Kang and Sang Gi Paik},
  journal={Veterinary parasitology},
  volume={111 4},
Theileria sergenti is the causative agent of persistent theileriosis in cattle. The ubiquitous infection of theileiriosis causes chronic anemia and fever in cattle, especially in exogenous cattle. In this study, we applied real-time polymerase chain reaction (PCR) for the diagnosis and quantification of parasite using specific primers for 33 kDa gene fragment of T. sergenti. Comparison of TaqMan PCR with traditional microscopic method, Giemsa's staining, on blood collected from cattle revealed… CONTINUE READING

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