Diabetes Induced Changes in Podocyte Morphology and Gene Expression Evaluated Using GFP Transgenic Podocytes

@inproceedings{Xu2016DiabetesIC,
  title={Diabetes Induced Changes in Podocyte Morphology and Gene Expression Evaluated Using GFP Transgenic Podocytes},
  author={Jianxiang Xu and Shirong Zheng and Patricia M. Kralik and Laxminarayanan Krishnan and Hui Huang and James B. Hoying and Lu Cai and Edward C. Carlson and Yi Tan and P N Epstein},
  booktitle={International journal of biological sciences},
  year={2016}
}
  • Jianxiang Xu, Shirong Zheng, +7 authors P N Epstein
  • Published in
    International journal of…
    2016
  • Biology, Medicine
  • The effect of diabetes in vivo has not been examined on isolated podocytes. To achieve this, GFP was expressed constitutively in podocytes of PGFP transgenic mice which were bred to OVE mice to produce diabetic OVE-GFP mice. Viewing GFP fluorescence, foot processes of OVE-GFP podocytes were visually and measurably effaced, which did not occur with less severe STZ diabetes. Over 300,000 podocytes were purified from each PGFP mouse but only 49,000 podocytes per diabetic OVE-GFP mouse. The low… CONTINUE READING

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