Dextran sodium sulfate inhibition of real-time polymerase chain reaction amplification: a poly-A purification solution.

@article{Kerr2012DextranSS,
  title={Dextran sodium sulfate inhibition of real-time polymerase chain reaction amplification: a poly-A purification solution.},
  author={Thomas A. Kerr and Matthew A. Ciorba and H. Matsumoto and Victoria R Davis and Jean J Luo and Scot Kennedy and Youhua Xie and Anisa Shaker and Brian K. Dieckgraefe and Nicholas O. Davidson},
  journal={Inflammatory bowel diseases},
  year={2012},
  volume={18 2},
  pages={
          344-8
        }
}
BACKGROUND Dextran sulfate sodium (DSS) induces experimental colitis and promotes colitis-associated cancer in rodents. Here we document potent inhibition of real-time quantitative polymerase chain reaction (qPCR) using cDNA from DSS-exposed mouse tissues, which complicates gene expression analysis. METHODS We characterize DSS inhibition of qPCR in-vitro and in a wide array of murine tissues following ingestion of DSS. We examine different approaches to RNA purification prior to cDNA… CONTINUE READING

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