Developments in Directed Evolution for Improving Enzyme Functions

@article{Sen2007DevelopmentsID,
  title={Developments in Directed Evolution for Improving Enzyme Functions},
  author={Sagar Sen and Veeranki Venkata Dasu and Bapi Mandal},
  journal={Applied Biochemistry and Biotechnology},
  year={2007},
  volume={143},
  pages={212-223}
}
The engineering of enzymes with altered activity, specificity, and stability, using directed evolution techniques that mimic evolution on a laboratory timescale, is now well established. In vitro recombination techniques such as DNA shuffling, staggered extension process (StEP), random chimeragenesis on transient templates (RACHITT), iterative truncation for the creation of hybrid enzymes (ITCHY), recombined extension on truncated templates (RETT), and so on have been developed to mimic and… Expand
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