Developmentally regulated mRNAs in 3T3-adipocytes: analysis of transcriptional control

@article{Cook1985DevelopmentallyRM,
  title={Developmentally regulated mRNAs in 3T3-adipocytes: analysis of transcriptional control},
  author={Karen Steinmiller Cook and Clayton R. Hunt and Bruce M. Spiegelman},
  journal={The Journal of Cell Biology},
  year={1985},
  volume={100},
  pages={514 - 520}
}
We have investigated the regulation of mRNA synthesis during 3T3-adipocyte differentiation by measuring the transcription of specific genes in isolated preadipocyte and adipocyte nuclei. Transcription was assayed by hybridization of newly synthesized RNA to cDNA clones coding for glycerophosphate dehydrogenase (GPD), the induced protein of 13K which is shown here to be related to myelin protein P-2, the induced protein of 28K, actin, and two RNAs that are not developmentally regulated… CONTINUE READING

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