Developmentally regulated expression of a cell surface class I nuclease in Leishmania mexicana.

  title={Developmentally regulated expression of a cell surface class I nuclease in Leishmania mexicana.},
  author={Will Sopwith and Alain Debrabant and Mat Yamage and Dennis M. Dwyer and Paul A. Bates},
  journal={International journal for parasitology},
  volume={32 4},

Molecular and Functional Analyses of a Novel Class I Secretory Nuclease from the Human Pathogen, Leishmania donovani*

It is hypothesized that within the micro-environments of its host, this leishmanial “secretory” nuclease could function at a distance away from the parasite to harness (i.e. hydrolyze/access) host-derived nucleic acids to satisfy the essential purine requirements of these organisms.

Molecular Characterization, Expression, and in Vivo Analysis of LmexCht1

The results indicate that the LmexCht1-chimera afforded a definitive survival advantage to the parasite within these mammalian hosts and could potentially represent a new virulence determinant in the mammalian phase of this important human pathogen.

Ecto-nucleotidases and Ecto-phosphatases from Leishmania and Trypanosoma parasites.

The present chapter provides an overview of recent discoveries related to the occurrence of ecto-nucleotidase and ecto -phosphatase activities in Leishmania and Trypanosoma parasites.

Stage-Specific Class I Nucleases of Leishmania Play Important Roles in Parasite Infection and Survival

The present review aims to present and discuss the roles played by different class I nucleases during the Leishmania lifecycle, especially regarding the establishment of mammalian host infection.

3′-Nucleotidase/Nuclease Activity Allows Leishmania Parasites To Escape Killing by Neutrophil Extracellular Traps

Results show that Leishmania infantum induces NET release and that promastigotes can escape NET-mediated killing by 3′-nucleotidase/nuclease activity, thus ascribing a new function to this enzyme.

Molecular Cloning and Characterization of P4 Nuclease from Leishmania infantum

Parasite of the genus Leishmania is reliant on the salvage pathway for recycling of ribonucleotides. A class I nuclease enzyme also known as P4 nuclease is involved in salvage of purines in cutaneous

Molecular Characterization of a Hyperinducible, Surface Membrane-anchored, Class I Nuclease of a Trypanosomatid Parasite*

Results of the nuclear run-on analyses demonstrated that the dramatic up-expression of 3′-NT/NU activity in response to purine starvation of C. luciliae was concomitant with the ∼100-fold elevation in steady-state mRNA specific for this gene.

The immunologically protective P-4 antigen of Leishmania amastigotes. A developmentally regulated single strand-specific nuclease associated with the endoplasmic reticulum.

The purified membrane-associated Leishmania pifanoi amastigote protein P-4 has been shown to induce protective immunity against infection and to elicit preferentially a T helper 1-like response in

Characterization of developmentally-regulated nucleases in promastigotes and amastigotes of Leishmania mexicana.

    P. Bates
    FEMS microbiology letters
  • 1993
The parasitic protozoon Leishmania mexicana was examined for the presence of 3'-nucleotidase/nuclease using substrate SDS-PAGE and the 29/31 kDa form showed a broader, unique substrate specificity in that it was also capable of digesting double-stranded RNA and DNA.

Dissection of the Functional Domains of theLeishmania Surface Membrane 3′-Nucleotidase/Nuclease, a Unique Member of the Class I Nuclease Family*

Some of the functional domains of this unique member of the class I nuclease family, the 3′-nucleotidase/nuclease (Ld3′NT/NU), are defined using the fidelity of a homologous expression system.