Developmental validation of the SPERM HY-LITER™ kit for the identification of human spermatozoa in forensic samples.

@article{Miller2011DevelopmentalVO,
  title={Developmental validation of the SPERM HY-LITER™ kit for the identification of human spermatozoa in forensic samples.},
  author={Kevin W. P. Miller and Jennifer B. Old and Brian R. Fischer and Brett A Schweers and Simona Stipinaite and Karl A. Reich},
  journal={Journal of forensic sciences},
  year={2011},
  volume={56 4},
  pages={
          853-65
        }
}
With sexual assault evidence, the visualization of spermatozoa confirms that ejaculation has occurred. However, microscopic examination of spermatozoa is a laborious process and can sometimes result in sperm cells being overlooked. Here, we present the developmental validation of the SPERM HY-LITER™ kit, which contains a human sperm-specific mouse monoclonal antibody coupled to a fluorescent Alexa 488 dye. The kit was tested using samples of human semen, saliva, blood, and urine, various animal… CONTINUE READING

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