Developmental potential of bovine hand-made clone embryos reconstructed by aggregation or fusion with distinct cytoplasmic volumes.

@article{Ribeiro2009DevelopmentalPO,
  title={Developmental potential of bovine hand-made clone embryos reconstructed by aggregation or fusion with distinct cytoplasmic volumes.},
  author={Eduardo de Souza Ribeiro and R. R. Gerger and Lain Uriel Ohlweiler and Ivens Ortigari and Joana Cl{\'a}udia Mezzalira and Fabiana Forell and Luciana Relly Bertolini and Jos{\'e} Luiz Cabreira Rodrigues and Carlos E Ambr{\'o}sio and Maria Angelica Miglino and A. Lorenzo Mezzalira and Marcelo Bertolini},
  journal={Cloning and stem cells},
  year={2009},
  volume={11 3},
  pages={
          377-86
        }
}
Animal cloning has been associated with developmental abnormalities, with the level of heteroplasmy caused by the procedure being one of its potential limiting factors. The aim of this study was to determine the effect of the fusion of hemicytoplasts or aggregation of hemiembryos, varying the final cytoplasmic volume, on development and cell density of embryos produced by hand-made cloning (HMC), parthenogenesis or by in vitro fertilization (IVF). One or two enucleated hemicytoplasts were… CONTINUE READING

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