Developmental expression of genes in chick growth cartilage detected byin situ hybridization


We have usedin situ hybridization to examine expression of collagen type I, II, and X mRNA and osteonectin mRNA in the chick epiphysis. Tissue samples from the proximal tibial growth cartilage were fixed in modified Carnoy's solution, dehydrated in ethanol, and embedded in paraffin. Longitudinal and transverse sections were dimineralized with HCl and… (More)
DOI: 10.1007/BF02556062


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