Developmental and hormonal regulation of direct shoot organogenesis and somatic embryogenesis in sugarcane (Saccharum spp. interspecific hybrids) leaf culture

@article{Lakshmanan2006DevelopmentalAH,
  title={Developmental and hormonal regulation of direct shoot organogenesis and somatic embryogenesis in sugarcane (Saccharum spp. interspecific hybrids) leaf culture},
  author={Prakash Lakshmanan and Robert J. Geijskes and Lifang Wang and Adrian D Elliott and Christopher P L Grof and Nils Berding and Grant R. Smith},
  journal={Plant Cell Reports},
  year={2006},
  volume={25},
  pages={1007-1015}
}
Rapid and efficient in vitro regeneration methods that minimise somaclonal variation are critical for the genetic transformation and mass propagation of commercial varieties. Using a transverse thin cell layer culture system, we have identified some of the developmental and physiological constraints that limit high-frequency regeneration in sugarcane leaf… CONTINUE READING