Developmental ability of porcine oocytes matured and fertilized in vitro.

@article{Funahashi1994DevelopmentalAO,
  title={Developmental ability of porcine oocytes matured and fertilized in vitro.},
  author={H Funahashi and Thomas Stumpf and S L Terlouw and Tom C Cantley and August Rieke and Billy Neil Day},
  journal={Theriogenology},
  year={1994},
  volume={41 7},
  pages={1425-33}
}
The developmental abilities of porcine oocytes matured and fertilized in vitro were examined in vivo and in vitro. Cumulus-oocyte complexes were cultured in mM199 supplemented with 10% porcine follicular fluid (PFF) and hormonal supplements (PMSG, hCG and estradiol-17beta) for 20 h and then without hormonal supplements for an additional 20 h. In Experiment 1, oocytes were then co-cultured for 6 h with spermatozoa which had been preincubated with 1% PFF (PFF-treated) or without (control… CONTINUE READING
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