Developmental ability of in vitro matured sheep oocytes collected during the nonbreeding season and fertilized in vitro with frozen ram semen.

@article{Pugh1991DevelopmentalAO,
  title={Developmental ability of in vitro matured sheep oocytes collected during the nonbreeding season and fertilized in vitro with frozen ram semen.},
  author={Philip J. A. Pugh and Y. Fukui and H Robin Tervit and Jeremy G. Thompson},
  journal={Theriogenology},
  year={1991},
  volume={36 5},
  pages={
          771-8
        }
}
During the nonbreeding season, oocytes recovered from ovaries of FSH-primed or nonprimed ewes were matured in the presence or absence of granulosa cells collected from ovaries of primed or nonprimed ewes prior to in vitro fertilization with either fresh or frozen-thawed sperm. Following fertilization, ova were cultured for 24 h in synthetic oviduct fluid medium (SOF) supplemented with 20% human serum at 39 degrees C under humidified 5% CO(2), 5% O(2), 90% N(2) and then assessed for cleavage… CONTINUE READING
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