Developmental Regulation of mRNAs Encoding Rat Brain Kainate/AMPA Receptors: A Northern Analysis Study

  title={Developmental Regulation of mRNAs Encoding Rat Brain Kainate/AMPA Receptors: A Northern Analysis Study},
  author={Guylaine M. Durand and R. Suzanne Zukin},
  journal={Journal of Neurochemistry},
Functionally diverse kainate/α‐amino‐3‐hydroxy‐5‐methyl‐4‐isoxazolepropionic acid (AMPA) receptors are generated by assembly of glutamate receptor (GluR)1, 2, and 3 subunits into homomeric and heteromeric channels. We examined GluR1, 2, and 3 gene expression in embryonic, neonatal, and adult rat brain by northern analysis under conditions of high stringency. In the adult, hybridization to a GluR1 riboprobe revealed the presence of an abundant RNA species, 5.2 kb in size, and minor bands of 3.2… 

Stoichiometries of AMPA receptor subunit mRNAs in rat brain fall into discrete categories

The present data suggest that forebrain AMPA‐type glutamate receptors can be classified into a limited number of recurring types, and are suggested that AR‐1,2 and AR‐2, the most closely related categories in clustering space, are largely telencephalic receptors.

The Postnatal Development of AMPA Receptor Subunits in the Basal Ganglia of the Rat

Observed changes in the expression of the AMPA receptor transcripts indicate that there are fundamental differences in theexpression of these receptor subunits in adults and neonates, contributing to differences in susceptibility to injury and disease in the aging brain.

A Novel Two‐Site Enzyme Immunoassay Reveals the Regional Distributions of and Developmental Changes in GluR1 and NMDAR1 Protein Contents in the Rat Brain

This two‐site enzyme immunoassay is a specific and unique method that enables us to measure absolute tissue contents of the glutamate receptors and will lead to further important discoveries on the biochemical alterations of these receptors.

Analysis of AMPA receptor properties during postnatal development of mouse hippocampal astrocytes.

Changes in astrocytic AMPA-R functioning early in postnatal development are demonstrated, although after P12 the receptor properties remained almost constant, which could hint at a developmental regulation of RNA editing in the arginine/glycine site.

Activity regulates the expression of AMPA receptor subunit GluR4 in developing visual cortex

  • Y. Akaneya
  • Biology, Chemistry
    The European journal of neuroscience
  • 2007
The results suggest that neuronal activity such as visual experience may be involved in the mechanism of the expression of GluR4, which is mediated by NMDAR and tuned by certain protein kinases at an early developmental stage in the visual cortex.

Molecular Determinants Controlling Functional Properties of AMPARs and NMDARs in the Mammalian CNS

Considering all combinatorial possibilities, the molecular diversity of glutamate-receptor channels is considerable (Hollmann, this volume).



Differential expression of three glutamate receptor genes in developing rat brain: an in situ hybridization study.

The finding of differential developmental regulation of the GluR-1, -2, and -3 genes indicates that the receptors they encode may have different influences on synaptic plasticity, neuronal survival, and susceptibility to excitatory amino acid toxicity.

Molecular cloning and functional expression of glutamate receptor subunit genes.

Three closely related genes, GluR1, GluR2, and GluR3, encode receptor subunits for the excitatory neurotransmitter glutamate. The proteins encoded by the individual genes form homomeric ion channels

Cloning of a cDNA for a glutamate receptor subunit activated by kainate but not AMPA

The cloning and expression of a functional rat glutamate receptor subunit cDNA, GluR6, which has a very different pharmacology from that of the GluLl–GluR4 class and exhibits an outwardly rectifying current–voltage relationship.

Cloning of a putative high-affinity kainate receptor expressed predominantly in hippocampal CA3 cells

A new member of the rat excitatory amino-acid receptor gene family, KA-1, that has a 30% sequence similarity with the previously characterized α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) receptor subunits GluR-A to -D3–5 is described.

A family of AMPA-selective glutamate receptors.

Four cloned cDNAs encoding 900-amino acid putative glutamate receptors with approximately 70 percent sequence identity were isolated from a rat brain cDNA library. In situ hybridization revealed

Flip and flop: a cell-specific functional switch in glutamate-operated channels of the CNS.

These results identify a switch in the molecular and functional properties of glutamate receptors operated by alternative splicing.