Developmental Expression of PEPT1 and PEPT2 in Rat Small Intestine, Colon, and Kidney

@article{Shen2001DevelopmentalEO,
  title={Developmental Expression of PEPT1 and PEPT2 in Rat Small Intestine, Colon, and Kidney},
  author={Hong Shen and David E Smith and F. Brosius},
  journal={Pediatric Research},
  year={2001},
  volume={49},
  pages={789-795}
}
Mammalian peptide transporters (PEPT1 and PEPT2) play a pivotal role in the absorption of small peptides from the intestine and kidney, respectively, and in the disposition and targeting of peptide or mimetic drugs. However, there are few reports on the molecular basis of their regulation, especially in the young. The aim of this study was to determine the developmental expression of intestinal and renal oligopeptide transporters in rats from embryonic to adult ages. Intestinal segments were… Expand
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References

SHOWING 1-10 OF 33 REFERENCES
Functional and molecular expression of intestinal oligopeptide transporter (Pept-1) after a brief fast.
TLDR
It is concluded that 1 day of fasting increases dipeptide transport in rat intestine by increasing the population of Pept-1 in the brush-border membrane, and the mechanism appears to be an increase in PePT-1 gene expression. Expand
Localization of PEPT1 and PEPT2 proton-coupled oligopeptide transporter mRNA and protein in rat kidney.
TLDR
The results conclusively demonstrate that although PEPT1 is expressed in early regions of the proximal tubule (pars convoluta), P EPT2 is specific for the latter regions of proximal Tubule ( pars recta). Expand
The oligopeptide transporter (Pept-1) in human intestine: biology and function.
  • S. Adibi
  • Biology, Medicine
  • Gastroenterology
  • 1997
TLDR
These studies suggest that the insulin stimulates dipeptide transport by increasing membrane insertion of oligopeptide transporter from a preformed cytoplasmic pool, and cholera toxin decreases dipeptic transport by inhibiting the activity of Pept-1 through an increase in the intracellular concentration of adenosine 3',5'-cyclic monophosphate. Expand
Hormonal regulation of oligopeptide transporter pept-1 in a human intestinal cell line.
TLDR
Results show that insulin, when it binds to its receptor, stimulates Gly-Gln uptake by Caco-2 cells by increasing the membrane population of Pept-1, the mechanism appears to be increased translocation of this transporter from a preformed cytoplasmic pool. Expand
Valacyclovir: a substrate for the intestinal and renal peptide transporters PEPT1 and PEPT2.
TLDR
It is concluded that valacyclovir is a substrate for the peptide transporters PEPT1 and P EPT2 and that a peptide bond is not a prerequisite for recognition as a substrate by the peptides transporter. Expand
Renal assimilation of oligopeptides: physiological mechanisms and metabolic importance.
  • S. Adibi
  • Chemistry, Medicine
  • The American journal of physiology
  • 1997
TLDR
The nutritional and metabolic regulation of renal assimilation of oligopeptides is suggested by the selective decrease in dipeptide balance across the kidneys of starved human subjects and by the insulin stimulation of diPEptide transport by a renal cell line. Expand
Immuno-localization of H+/peptide cotransporter in rat digestive tract.
TLDR
Observations show that PepT1 is specific to the brush border of the differentiated absorptive epithelial cells and suggest that H+-coupled uptake of small peptides and peptide-like drugs occurs at the apical membrane of these cells in the small intestine. Expand
Development of Dipeptide Transport in the Intestinal Mucosa of Rabbits
TLDR
The results indicate that a very efficient dipeptide influx process occurs in the newborn rabbit, and the developmental patterns of the transport systems for Gly-Pro and for free glycine are different, which provides ontogenetic evidence for the distinction between diPEptide and free amino acid carrier systems. Expand
Peptide transporters in the intestine and the kidney.
TLDR
C cultured cell lines of intestinal and renal origin that express PEPT 1 and P EPT 2 have been identified and are likely to facilitate studies on the regulatory aspects of the peptide transporters. Expand
Sequence, tissue distribution and developmental changes in rat intestinal oligopeptide transporter.
TLDR
Rat PepT1 mRNA levels were highest in 4-day old rats, and then decreased reaching the adult level by day 28 after birth, indicating that the expressions of PepT 1 gene change markedly during development. Expand
...
1
2
3
4
...