Development of yeast strains for the efficient utilisation of starch: evaluation of constructs that express α-amylase and glucoamylase separately or as bifunctional fusion proteins

Abstract

Eight constructions involving the Bacillus subtilis α-amylase gene (amyE), a mouse pancreatic α-amylase cDNA (AMY2) and an Aspergillus awamori glucoamylase cDNA (glaA) were prepared: three fusion genes, involving one α-amylase and the glucoamylase, two double-cassette plasmids (expressing one or other α-amylase and the glucoamylase) and three single… (More)
DOI: 10.1007/BF00166927

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