Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses

@article{BellauPujol2005DevelopmentOT,
  title={Development of three multiplex RT-PCR assays for the detection of 12 respiratory RNA viruses},
  author={S Bellau-Pujol and Astrid Vabret and L. Legrand and Julia Dina and Stéphanie Gouarin and Jo{\"e}lle Petitjean-Lecherbonnier and Bruno Pozzetto and Christophe Ginevra and François Freymuth},
  journal={Journal of Virological Methods},
  year={2005},
  volume={126},
  pages={53 - 63}
}

Figures and Tables from this paper

A multiplex one-tube nested real time RT-PCR assay for simultaneous detection of respiratory syncytial virus, human rhinovirus and human metapneumovirus
TLDR
mOTNRT-PCR assay reveals extremely higher sensitivity than that of RT-q PCR assay for detecting RSV, HRV and HMPV in clinical settings, compared with traditional two-step nested PCR assay.
Comparison of Real-Time PCR Assays with Fluorescent-Antibody Assays for Diagnosis of Respiratory Virus Infections in Children
TLDR
The PCR assays were significantly more sensitive than FA assays for detecting respiratory viruses, especially parainfluenza virus and adenovirus, and will lead to improved diagnosis of respiratory illness in children.
In-house standardization and validation of a multiplex RT-PCR assay for the detection of 13 respiratory viruses
TLDR
This multiplex RT-PCR format shows an adequate efficiency, demonstrating an excellent sensitivity, specificity and repeatability for all the studied respiratory viruses.
Comparison of molecular assays for the rapid detection and simultaneous subtype differentiation of the pandemic influenza A (H1N1) 2009 virus.
TLDR
The results show that, although rRTPCR had the advantage, the detection rates of the molecular assays varied depending upon the source of the influenza A (H1N1)v virus, and suggest that rRT-PCR sometimes detected virus in extremely low abundance and thus required validation of analytical performance and clinical correlation.
Performance of a rapid molecular multiplex assay for the detection of influenza and picornaviruses
TLDR
The Luminex xTAG Respiratory Viral Panel RVP Fast assay detected clinically important viruses in a single genomic test and will thus be useful for detecting several viruses causing respiratory tract disorders.
Rhinovirus detection by real-time RT-PCR in children with acute respiratory infection in Buenos Aires, Argentina.
TLDR
The utility and clinical sensitivity of an adapted real-time RT-PCR assay for HRV detection and the frequency of HRV in respiratory samples from children under 2 years of age, with acute respiratory infection (ARI), from Buenos Aires, Argentina are described.
...
...

References

SHOWING 1-10 OF 67 REFERENCES
Evaluation of a multiplex reverse transcriptase PCR ELISA for the detection of nine respiratory tract pathogens.
  • W. Puppe, J. Weigl, J. Groen
  • Biology
    Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
  • 2004
Comparison of a Multiplex Reverse Transcription-PCR-Enzyme Hybridization Assay with Conventional Viral Culture and Immunofluorescence Techniques for the Detection of Seven Viral Respiratory Pathogens
TLDR
It is suggested that this multiplex RT-PCR-EHA provides a highly sensitive and specific means of diagnostic detection of major respiratory viruses.
Simultaneous detection of fourteen respiratory viruses in clinical specimens by two multiplex reverse transcription nested‐PCR assays
TLDR
Both multiplex RT‐PCR assays provide a system capable of detecting and identifying simultaneously 14 different respiratory viruses in clinical specimens with high sensitivity and specificity, being useful for routine diagnosis and survey of these viruses within the population.
Comparison of three non-nested RT-PCR for the detection of influenza A viruses.
  • A. Vabret, G. Sapin, F. Freymuth
  • Biology, Medicine
    Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology
  • 2000
Rapid and Sensitive Method Using Multiplex Real-Time PCR for Diagnosis of Infections by Influenza A and Influenza B Viruses, Respiratory Syncytial Virus, and Parainfluenza Viruses 1, 2, 3, and 4
TLDR
A rapid real-time multiplex PCR assay was developed for the detection of influenza A and influenza B viruses, human respiratory syncytial virus (RSV), parainfluenza virus 1 (PIV1), PIV2, PIV3, and PIV4 in a two-tube multiplex reaction which used molecular beacons to discriminate the pathogens.
Detection of respiratory syncytial virus by reverse transcription-PCR and hybridization with a DNA enzyme immunoassay
Nasal aspirates from 238 infants hospitalized with acute respiratory infections during the winter of 1994 and 1995 were tested for respiratory syncytial virus (RSV) by immunofluorescence assay (IFA)
Simultaneous detection of influenza A, B, and C viruses, respiratory syncytial virus, and adenoviruses in clinical samples by multiplex reverse transcription nested‐PCR assay
TLDR
A new multiplex reverse transcription nested‐polymerase chain reaction (RT‐PCR) assay that is able to detect and type different respiratory viruses simultaneously is described and demonstrates the use of this method for both laboratory routine diagnosis and surveillance of all these viruses.
Simultaneous Detection and Identification of Human Parainfluenza Viruses 1, 2, and 3 from Clinical Samples by Multiplex PCR
TLDR
Multiplex RT-PCR assays that detect and differentiate HPIV serotypes 1, 2, and 3 in a combined reaction are described, providing sensitive, specific, and simplified tools for the rapid diagnosis of HPIV infections.
Direct Detection of Respiratory Syncytial Virus, Parainfluenza Virus, and Adenovirus in Clinical Respiratory Specimens by a Multiplex Reverse Transcription-PCR Assay
  • C. Osiowy
  • Biology, Medicine
    Journal of Clinical Microbiology
  • 1998
TLDR
The multiplex RT-PCR assay can be used as a rapid and sensitive diagnostic method for major respiratory viruses, and was found to be specific for all 12 prototype viruses tested, with no interassay cross amplification or amplification of other respiratory viruses.
Rapid simultaneous diagnosis of infections with respiratory syncytial viruses A and B, influenza viruses A and B, and human parainfluenza virus types 1, 2, and 3 by multiplex quantitative reverse transcription-polymerase chain reaction-enzyme hybridization assay (Hexaplex).
  • J. Fan, K. Henrickson, L. Savatski
  • Biology, Medicine
    Clinical infectious diseases : an official publication of the Infectious Diseases Society of America
  • 1998
TLDR
All eight "false-positive" Hexaplex results (in comparison with negative viral culture results) were for symptomatic patients with low numbers of virus RNA copies, suggesting that HexapLex may be more sensitive than virus culture.
...
...