Development of thermodynamic models for simulating probe dissociation profiles in fluorescence in situ hybridization.

@article{Yilmaz2007DevelopmentOT,
  title={Development of thermodynamic models for simulating probe dissociation profiles in fluorescence in situ hybridization.},
  author={Luftu Safak Yilmaz and Daniel R. Noguera},
  journal={Biotechnology and bioengineering},
  year={2007},
  volume={96 2},
  pages={349-63}
}
Stringency in ribosomal RNA (rRNA)-targeted fluorescence in situ hybridization (FISH) is typically adjusted with formamide, and the optimum formamide concentration at which the probe can hybridize with the target rRNA, but not with rRNAs with mismatches, is to be found experimentally. This is a difficult task when target or closest non-target organisms are not available in pure culture, or when there are numerous non-targets of concern. The objective of this work was to formulate mechanistic… CONTINUE READING
10 Citations
0 References
Similar Papers

Citations

Publications citing this paper.
Showing 1-10 of 10 extracted citations

Similar Papers

Loading similar papers…